The obligate intracellular parasite Plasmodium falciparum, an agent of human malaria, relies on membrane transport proteins for the uptake of nutrients from the host, disposal of metabolic waste, exchange of metabolites between organelles, and generation and maintenance of transmembrane electrochemical gradients for its growth and replica-tion within human erythrocytes. Membrane transport proteins perform crucial roles in cell physiology. Here the authors introduce QUEEN, a framework to describe and share DNA materials and construction protocols. DNA constructs and their annotated sequence maps have been rapidly accumulating with the advancement of DNA cloning, synthesis, and assembly methods. We propose QUEEN as a solution to start significantly advancing the material and protocol sharing of DNA resources. QUEEN-generated GenBank files are compatible with existing DNA repository services and software.
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A GenBank file generated by QUEEN can regenerate the process code such that it perfectly clones itself and bequeaths the same process code to its successive GenBank files, recycling its partial DNA resources. QUEEN enables the flexible design of new DNA by using existing DNA material resource files and recording its construction process in an output file (GenBank file format). Here, we report a framework QUEEN (framework to generate quinable and efficiently editable nucleotide sequence resources) to resolve these issues and accelerate the building of DNA. Furthermore, the use of previously developed DNA materials and building protocols is usually not appropriately credited. However, as commonly seen in the life sciences, no framework exists to describe reproducible DNA construction processes. Such resources have also been utilized in designing and building new DNA materials.
equi infection diagnosis, as well as helping in treatment selection.ĭNA constructs and their annotated sequence maps have been rapidly accumulating with the advancement of DNA cloning, synthesis, and assembly methods. Our new quantitative PCR is a trustable tool that will improve the speed of R. Furthermore, it did not present any cross-reaction with normal flora from the equine respiratory tract, nor commonly encountered respiratory pathogens in horses or other genetically close organisms. Additionally, our multiplex qPCR shows high specificity in in-silico analysis. equi housekeeping gene choE and macrolide resistance genes erm(46) and erm(51) showed high analytic sensitivity with a limit of detection (LOD) individually and in combination below 12 complete genome copies per PCR reaction, and an amplification efficiency between 90% and 147%. The three sets of oligos designed in this study to identify R.
equi and related macrolide resistance genes in equine respiratory samples. Here, we have developed and analytically validated a multiplex qPCR for the simultaneous detection of R. equi to current antimicrobial treatment, new tools that can provide a fast and accurate diagnosis of the disease and antimicrobial resistance profile are needed. equi is an important veterinary pathogen that takes the lives of many foals every year.
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